Lemuru Fish (Sardinella Lemuru) Oil effects on Body Weight of Wistar Rats Induced by Trans Fatty Acids
Abstract
The content of EPA and DHA in lemuru fish oil can be used to prevent obesity risk factors. This study aims to determine the effect of purified lemuru fish oil on the weight of Wistar rats induced by trans fatty acids. This research is an experimental study using a randomized block design with a post test only control group. The research sample was 30 male Wistar rats aged two to three months, grouped into two control groups (K) which were given standard feed (K0) and those given standard feed after two weeks of trans fatty acid induction (K1) and three treatment groups (P). ) with lemuru fish oil intervention as much as 0.34 ml (P1), 0.67 ml (P2), and 1.34 ml (P3). The induction material was 2.3 g of liquid margarine, containing 3.8% trans fatty acids. The intervention material was lemuru fish oil from the purification process containing 18.05% EPA and 9.37% DHA. Induction of margarine for two weeks made the body weight of the rats increased by 27.90% (P2) compared to the control (K0) 16.06%. After the intervention period of lemuru fish oil for three weeks, the weight of the treatment group P1 (18.53%), P2 (14.74%), and P3 (13.19%) was lower than the control group K0 (22.79%) and K1 (25.31%).
Keywords
References
AKG, 2019. (2019). Permenkes Nomor 28 Tahun 2019. Kementerian Kesehatan Republik Indonesia.
Ali, A., Amalia, L., & Suptijah, P. (2015). Pemberian Kitosan dan Pengaruhnya Terhadap Berat Badan dan Kadar Trigliserida Darah Tikus Sprague-Dawley Yang Diberi Pakan Asam Lemak Trans. Jurnal Gizi dan Pangan, 10(1), 9–16. https://doi.org/10.25182/jgp.2015.10.1.%p
Almatsier, S. (2011). Prinsip Dasar Ilmu Gizi. Gramedia Pustaka Utama.
Al-Qahtani, A. M. (2019). Prevalence and Predictors of Obesity and Overweight among Adults Visiting Primary Care Settings in the Southwestern Region, Saudi Arabia. BioMed Research International, 2019, 8073057. https://doi.org/10.1155/2019/8073057
Andhikawati, A., Permana, R., & Akbarsyah, N. (2020). Karakteristik Minyak Ikan Lemuru Yang Disimpan Selama 30 Hari Pada Suhu Rendah (5°C). J Akuatek, 1(1), 46–52.
Boahen, Y., Azumah, S., Apanyin, S., Novick, B., & Wubah, D. (2012). The Quality And Infrared Determination Of Trans Fatty Acid Contents In Some Edible Vegetable Oil. African Journal of Food Science and Technology, 3(6), 142–148.
BPS. (2020). Statistik Produksi Perikanan Laut. https://www.bps.go.id/subject/56/perikanan.html
Buckley, J. D., & Howe, P. R. C. (2010). Long-Chain Omega-3 Polyunsaturated Fatty Acids May Be Beneficial for Reducing Obesity—A Review. Nutrients, 2(12), 1212–1230. https://doi.org/10.3390/nu2121212
Christijani, R. (2019). Penentuan Diagnosis Sindrom Metabolik Berdasarkan Penilaian Skor Sindrom Metabolik dan NCEP ATP-III Pada Remaja [Penelitian di Beberapa SMA di Kota Bogor]. Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research), 42(1), 21–28. https://doi.org/10.22435/pgm.v42i1.2418
Dorfman, S. E., Laurent, D., Gounarides, J. S., Li, X., Mullarkey, T. L., Rocheford, E. C., Sari-Sarraf, F., Hirsch, E. A., Hughes, T. E., & Commerford, S. R. (2009). Metabolic Implications of Dietary Trans-fatty Acids. Obesity, 17(6), 1200–1207. https://doi.org/10.1038/oby.2008.662
EFSA, E. F. S. (2018). Scientific and technical assistance on trans fatty acids. EFSA Supporting Publications, 15(6), 1433E. https://doi.org/10.2903/sp.efsa.2018.EN-1433
Flachs, P., Rossmeisl, M., & Kopecky, J. (2014). The Effect of n-3 Fatty Acids on Glucose Homeostasis and Insulin Sensitivity. Physiological Research, S93–S118. https://doi.org/10.33549/physiolres.932715
Hermina, H., & Prihatini, S. (2016). Gambaran Konsumsi Sayur dan Buah Penduduk Indonesia dalam Konteks Gizi Seimbang: Analisis Lanjut Survei Konsumsi Makanan Individu (SKMI) 2014. Buletin Penelitian Kesehatan, 44(3), 205–218. https://doi.org/10.22435/bpk.v44i3.5505.205-218
Ibrahim, B., Suptijah, P., & Yogaswara, G. (2015). Karakterisasi Minyak Ikan Dari Hasil Samping Industri Penepungan Ikan Lemuru (Sardinella Lemuru) Dengan Metode Pemurnian Alkali. J Dinamika Maritim, 5(1), 1–7.
Kurniawan, S. C. ( 0810071 ). (2011). Efek Ekstrak Rimpang Jahe Merah (Zingiber officinale Linn.Var.rubrum) Terhadap Gambaran Histopatologi Ulkus Gaster Pada Mencit Galur Swiss Webster Jantan Yang Diinduksi Asetosal [Skripsi, Universitas Kristen Maranatha]. https://doi.org/10/0810071_References.pdf
Larasati, R., Wirjatmadi, B., & Adriani, M. (2017). Pengaruh Pemberian Trans Fatty Acid (Tfa) Dari Margarin Dan Minyak Kelapa Sawit Yang Dipanaskan Berulang Terhadap Kadar Glukosa Darah Puasa Pada Tikus Wistar. The Indonesian Journal of Public Health, 11(1), 69. https://doi.org/10.20473/ijph.v11i1.2016.69-77
Lee, T. C., Ivester, P., Hester, A. G., Sergeant, S., Case, L., Morgan, T., Kouba, E. O., & Chilton, F. H. (2014). The impact of polyunsaturated fatty acid-based dietary supplements on disease biomarkers in a metabolic syndrome/diabetes population. Lipids in Health and Disease, 13(1), 196. https://doi.org/10.1186/1476-511X-13-196
Lu, M., Sarruf, D. A., Talukdar, S., Sharma, S., Li, P., Bandyopadhyay, G., Nalbandian, S., Fan, W., Gayen, J. R., Mahata, S. K., Webster, N. J., Schwartz, M. W., & Olefsky, J. M. (2011). Brain PPAR-γ promotes obesity and is required for the insulin–sensitizing effect of thiazolidinediones. Nature Medicine, 17(5), 618–622. https://doi.org/10.1038/nm.2332
Maryanto, S. (2013). The effects of red guava (Psidium guajava L) fruits on lipid peroxidation in hypercholesterolemic rats. Basic Research Journal of Medicine and Clinical Sciences, 2(11), 116–121.
Maulana, I. T., Sukraso, & Damayanti, S. (2014). Kandungan Asam Lemak Dalam Minyak Ikan Indonesia. Jurnal Ilmu dan Teknologi Kelautan Tropis, 6(1), 121–130.
Mohammadnezhad, M., Alqahtani, N. S., Salusalu, M. V., & Konrote, A. (2017). Prevalence and determinant factors of overweight and obesity among Pacific people: A systematic review study. Global Advanced Research Journal of Medicine and Medical Sciences, 6(6), 91–102.
Muchtadi, D. (1989). Evaluasi Nilai Gizi Pangan. Institut Pertanian Bogor Press.
Nichols, P., McManus, A., Krail, K., Sinclair, A., & Miller, M. (2014). Recent Advances in Omega-3: Health Benefits, Sources, Products and Bioavailability. Nutrients, 6(9), 3727–3733. https://doi.org/10.3390/nu6093727
Nisa, F. Z., Probosari, E., & Fitranti, D. Y. (2017). Hubungan Asupan Omega-3 Dan Omega-6 Dengan Kadar Trigliserida Pada Remaja 15-18 Tahun. Journal of Nutrition College, 6(2), 191–197.
Poudyal, H., Panchal, S. K., Diwan, V., & Brown, L. (2011). Omega-3 fatty acids and metabolic syndrome: Effects and emerging mechanisms of action. Progress in Lipid Research, 50(4), 372–387. https://doi.org/10.1016/j.plipres.2011.06.003
Riskesdas, 2013. (2013). Hasil Utama Riskesdas 2013. Kementerian Kesehatan Republik Indonesia.
Rubio-Rodríguez, N., Beltrán, S., Jaime, I., de Diego, S. M., Sanz, M. T., & Carballido, J. R. (2010). Production of omega-3 polyunsaturated fatty acid concentrates: A review. Innovative Food Science & Emerging Technologies, 11(1), 1–12. https://doi.org/10.1016/j.ifset.2009.10.006
Ryan, K. K., Li, B., Grayson, B. E., Matter, E. K., Woods, S. C., & Seeley, R. J. (2011). A role for central nervous system PPAR-γ in the regulation of energy balance. Nature Medicine, 17(5), 623–626. https://doi.org/10.1038/nm.2349
Ryan, K. K., & Seeley, R. J. (2013). Food as a Hormone. Science, 339(6122), 918–919. https://doi.org/10.1126/science.1234062
Sacks, F. M., Lichtenstein, A. H., Wu, J. H. Y., Appel, L. J., Creager, M. A., Kris-Etherton, P. M., Miller, M., Rimm, E. B., Rudel, L. L., Robinson, J. G., Stone, N. J., Van Horn, L. V., & American Heart Association. (2017). Dietary Fats and Cardiovascular Disease: A Presidential Advisory From the American Heart Association. Circulation, 136(3), e1–e23. https://doi.org/10.1161/CIR.0000000000000510
Sarker, S. (2020). By-products of fish-oil refinery as potential substrates for biogas production in Norway: A preliminary study. Results in Engineering, 6, 100137. https://doi.org/10.1016/j.rineng.2020.100137
Trisviana, O. (2012). Pengaruh Pemberian Margarin terhadap Berat Badan dan Kadar Trigliserida Serum Tikus Sprague-dawley [Skripsi].
van Dijk, S. J., Feskens, E. J. M., Bos, M. B., Hoelen, D. W. M., Heijligenberg, R., Bromhaar, M. G., de Groot, L. C. P. G. M., de Vries, J. H. M., Müller, M., & Afman, L. A. (2009). A saturated fatty acid-rich diet induces an obesity-linked proinflammatory gene expression profile in adipose tissue of subjects at risk of metabolic syndrome. The American Journal of Clinical Nutrition, 90(6), 1656–1664. https://doi.org/10.3945/ajcn.2009.27792
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