Bacterial Contamination of Escherichia coli in Long Beans at Traditional Markets | Shodikin | Jurnal Kesehatan Prima
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Bacterial Contamination of Escherichia coli in Long Beans at Traditional Markets

Muhammad Ali Shodikin(1*), Ika Rahmawati Sutejo(2), Dini Agustina(3), Angga Mardro Raharjo(4), Anaditya Wahyu Kumudhaningsih(5)
(1) University of Jember
(2) University of Jember
(3) University of Jember
(4) University of Jember
(5) University of Jember
(*) Corresponding Author
DOI : 10.32807/jkp.v16i1.706

Abstract

Escherichia coli (E. coli) bacteria may contaminating uncooked food and cause food poisoning disease. Long beans (Vigna sinensis L) is one of the vegetable that consumed by people in raw (uncooked) and often traded in traditional markets. Many traditional markets do not meet sanitation requirements and many sellers do not perform good personal hygiene. These situation can potentially to cause bacterial contamination of vegetables sold in traditional markets. There has been no research examining E. coli contamination in long beans that sold at traditional markets in Jember.The purpose of this study to prove the contamination of E. coli bacteria in long beans at traditional markets in Jember. The research method uses an observational descriptive design with the cross sectional approach. Research places in eight traditional markets in Jember Regency and Microbiology Laboratory, Faculty of Medicine, University of Jember. The samples used amounted to 40 long beans purchased from 40 traditional market traders. The microbiological examination is performed using the most probable number (MPN) method, namely presumptive test and completed test. The results showed from the presumptive test all of 40 samples (100%) contaminated with coliform bacteria. After completed tests, 36 out of 40 samples (90%) were found contaminated by E. coli. The majority of long beans sold in traditional markets have been contaminated by E. coli bacteria.

Keywords


Long Beans; E. coli; Contamination

References


Addis, M., Sisay, D. A review on major food borne bacterial illnesses. Journal of Tropical Diseases. 2015; 3(4):1-7.

BPOM. Pedoman Penerapan Peraturan Badan POM tentang Cemaran Mikroba dalam Pangan Olahan. Direktorat Standardisasi Pangan Olahan Deputi Bidang Pengawasan Pangan Olahan Badan Pengawas Obat dan Makanan RI. BPOM. 2019; 1-33.

Djangaopa, Y., Manginsela, E. P., Baroleh, J. Kontribusi perempuan pedagang sayuran terhadap pendapatan keluarga di pasar bahu manado. Agri-SosioEkonomi Unsrat. 2018; 14: 45–54.

Francis, J., Kihla, T., Tatsinkou, B. F., Nkengfack, J. M. Bacterial and parasitic contaminants of salad vegetables sold in markets in Fako Division, Cameroon and evaluation of hygiene and handling practices of vendors. BMC Research Notes. 2018; 11(100):1–7.

Imran, A. N., Idrus, M. I., Kurniati. Pengaruh pemberiaan berbagai jenis pupuk kandang terhadap hasil produksi tanaman kacang panjang di kabupaten maros. Jurnal Agrotan. 2017; 3(2): 42–49.

Jang, J., Hur, H., Sadowsky, M. J., Byappanahalli, M. N., Yan, T., Ishii, S. Environmental Escherichia coli : ecology and public health implications — a review. Journal of Applied Microbiology 123. 2017; 570–581.

Le, H., Chau, Q., Thong, H. T., Chao, N. Van, Hoang, P., Hung, S., Hai, V. Van, & An, L. Van. Microbial and parasitic contamination on fresh vegetables sold in traditional markets in Hue City,. Journal of Food and Nutrition Research. 2014; 2(12): 959–964.

Li, W., Wu, S., Fu, P., Liu, J., Han, H., Bai, L., dkk. National molecular tracing network for foodborne disease surveillance in China. Food Control. 2018; 88: 28–32.

Luna-Guevara, J. J., Arenas-Hernandez, M. M. P., Martínez De La Peña, C., Silva, J. L., & Luna-Guevara, M. L. The role of pathogenic E. coli in fresh vegetables: behavior, contamination factors, and preventive measures. International Journal of Microbiology. 2019; 1–10.

Murray, P. R., Rosenthal, K. S., & Pfaller, M. A. (2016). Medical Microbiology. In Elsevier (8th ed., Vol. 51, Issue 1).

Pudjirahayu, A. (2017). Bahan Ajar Gizi: Pengawasan Mutu Pangan. In Bahan Ajar Gizi (pp. 1–311).

Purnama, S. G., Subrata, M. Hubungan higiene, fasilitas dan sanitasi lingkungan dengan kualitas mikrobilogi serta identifikasi Eschericia Coli O157: H7 pada sate languan. Jurnal Kesehatan Lingkungan Indonesia. 2019; 18(2): 104-112.

Ramadhani, N. R., Dian, L., Yuliawati, S. Kualitas bakteriologis berdasarkan keberadaan Salmonella sp pada selada (Lactusa sativa). Jurnal Kesmas Jambi. 2016; 1 (1):11–18.

Reswari, H. A., Syukur, M., & Suwarno, D. W. B. Kandungan antosianin dan karotenoid serta komponen produksi pada kacang panjang berpolong ungu dan hijau. Indonesian Journal of Agronomy. 2019; 47(1): 61–67.

Salamandane, C., Fonseca, F., Afonso, S., Lobo, M. L., Antunes, F., Matos, O. Handling of fresh vegetables: Knowledge, hygienic behavior of vendors, public health in maputo markets, Mozambique. International Journal of Environmental Research and Public Health. 2020; 17(17):1–17.

Syifaa, N., Ghazali, H., Haslindawaty, N., Rashid, A. Molecular identification of bacterial communities from vegetables samples as revealed by DNA sequencing of Universal Primer 16S rRNA gene. The International Journal of Medicine and Sciences. 2019: 4(1): 19–26.


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