Anti-Inflammatory of Papaya Leaf Extract (Carica Papaya L) Towards Membrane Stabilization of Red Blood Cells | Kamilla | Jurnal Kesehatan Prima

Anti-Inflammatory of Papaya Leaf Extract (Carica Papaya L) Towards Membrane Stabilization of Red Blood Cells

Laila Kamilla(1), Sri Tumpuk(2), Maulidiyah Salim(3*)
(1) Poltekkes Kemenkes Pontianak
(2) Poltekkes Kemenkes Pontianak
(3) Poltekkes Kemenkes Pontianak
(*) Corresponding Author
DOI : 10.32807/jkp.v15i1.399

Abstract

Traditional medical plants are known to society long ago. Apart from easily obtained and inexpensive, it can cure diseases with few side effects than modern medicine. Papaya leaves were used not only because of contained various chemical compounds with pharmacological effects but also alkaloids, flavonoids, saponins, and tannins compounds indicated as anti-inflammatory. This study applied a quasi-experimental design to test the papaya leaves anti-inflammatory activity. The red blood cell stabilization method was employed because analogous to the lysosomal membrane affected the inflammatory process. Purposive sampling was used, creating papaya leaves extract of 50 ppm, 100 ppm, 200 ppm, 400 ppm, 600 ppm, and 800 ppm concentration, made 24 total samples by four times replication. Based on the red blood cell lysis inhibition, the anti-inflammatory activity was measured and was compared with the positive control (diclofenac sodium). The papaya methanol extract result showed the highest anti-inflammatory activity at 800 ppm of 74.29%. The most effective concentration was at 200 ppm of 62.19%. Tukey's test showed p1.000 ≥ 0.05, suggesting H0 was accepted. There was no difference between the anti-inflammatory activity of papaya leaf methanol extract and diclofenac sodium showing stabilization of red blood cell membranes, indicating papaya potentially as an anti-inflammatory.

Keywords


Anti-inflammatory;Papaya Leaves;Membrane Stability;Erythrocytes

References


Avula, M., Pallu, R., & Reddy, G. V. (2010). Anti Inflammatory Activity of Flavonoid Fraction Isolated From the Stem Bark of Butea Monosperma (Lam): A Mechanism Based Study. International Journal of Phytopharmacology. 2010;1(2), 124–132.

Fadilla, P. Z. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirih (Piper betle L) Terhadap Propionibacterium acne dan Staphylococcus aureus Multiresisten. [skripsi] Universitas Muhammadiyah Surakarta. 2010.

Imaga, N. A., Gbenle, G. O., Okochi, V. I., Adenekan, S., Duro-Emmanuel, T., Oyeniyi, B., … Ekeh, F. C.Phytochemical and antioxidant nutrient constituents of Carica papaya and parquetina nigrescens extracts. Scientific Research and Essays. 2010;5(16), 2201–2205.

Karakaya, S., & El, S. N.Total Phenols and Antioxidant Activities of Some Herbal Teas and In Vitro Bioavailability of Black Tea Polyphenols. Journal of Agricultural Faculty of Gaziosmanpasa University. 2006;23(1), 1–8.

Kemenkes RI, Formularium Ramuan Obat Tradisional Indonesia. 2017.

Kumar, V., Bhat, Z. A., Kumar, D., Khan, N. A., &, & Chashoo, I. A. Evaluation of Anti-Inflammatory Potential of Leaf Extracts of Skimmia Anquetilia. Asian Pasific Journal of Tropical Biomedicine. 2012;2(8), 627–630.

Madhavi, P., Rao, M., Vakati, K., Rahman, H., & Eswaraiah, M. C.Evaluation of Anti-Inflammatory Activity of Citrullus lanatus Seed Oil by In-vivo and In-vitro Models. International Research Journal of Pharmaceutical Applied Science. 2012;2(4), 104–108.

Mahatriny, N. N., Payani, N. P. S., Oka, I. B. M., & Astuti, K. W. Skrining Fitokimia Ekstrak Etanol Daun Pepaya (Carica papaya L.) yang Diperoleh dari Daerah Ubud, Kabupaten Gianyar, Bali. Jurnal Farmasi Udayana. 2014;3(1), 8–13.

Mangan, Y. Solusi Sehat Mencegah & Mengatasi Kanker (Cetakan I). Jakarta: Agromedia Pustaka. 2009.

Middleton, E., Kandaswami, C., & Theoharides, T. C.The Effects Of Plant Flavonoids on Mammalian Cells: Implications For Inflammation, Heart Disease, And Cancer. Pharmacological Reviews. 2000;52(4), 673–751.

Nijveldt, R. J., Nood, E. van, Hoorn, D. E. van, Boelens, P. G., Norren, K. van, & Leeuwen, P. A. van. Flavonoid : A Review Of Propbable Mechanisms Of Action And Application. American Journal of Clinical Nutrition.2001;74, 418–425.

Notoadmojo, & Soekidjo. Metodologi Penelitian Kesehatan. Jakarta: Rineka Cipta. 2012.

OT, K., Akilbinu, M., AA Ayankunle, & EO Awe. Evaluation of (Anti-inflammatory And Antynociceptive Pontentials of Khaya senegalensis A Juss (Meliaceae) Stem Bark Aqueous Extract. British Journal of Medicine And Medical Research.2013;3(2), 216–229.

Owoyele, B. V., Adebukola, O. M., Funmilayo, A. A., & Soladoye, A. O. Anti-inflammatory Activities Of Ethanolic Extract of Carica Papaya Leaves. Journal Inflammopharmacology. 2008;16(4), 168–173. https://doi.org/10.1007/s10787-008-7008-0

Oyedapo, O., Akinpelu, B. A., Akinwunmi, K. F., Adeyinka, M. O., & Sipeolu, F. O. Red Blood Cell Membrane Stabilizing Potentials Of Extracts Of Lantana Camara And Its Fractions. International Journal of Plant Physiology and Biochemistry.2010;2(4), 46–51. Retrieved from http://www.academicjournals.org/ijppb/PDF/PDF 2010/Oct/Oyedapo et al.pdf

R, I., K, N., Tamil Selvi S, Bharathidasan R, & Rekha D. A Comparative Study of Anti-Inflammatory Activities of Certain Herbal Leaf Extracts. International Journal of Pharmacy and Integrated Life Sciences, 2013;1(2), 67–77.

Renata Ayuni. Khasiat Selangit Daun-Daun Ajaib Tumpas Beragam Penyakit (Cetakan I). Yogyakarta: Arasaka. 2012.

Setiawan Dalimartha. Ramuan Tradisional Untuk Pengobatan Kanker (Cetakan V). Jakarta: Penebar Swadaya. 2003.

Tanu, I., Syarif, A., Estuningtyas, A., Setiawati, A., Muchtar, A, H., & Arif, A. Farmakologi dan Terapi. Jakarta: Fakultas Kedokteran Universitas Indonesia. 2002.

Tim Cahaya. Kumpulan Obat Tradisional Nusantara (Cetakan II). Jakarta: Rama Edukasitama. 2011.


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