The Effectiveness of 80% Kefir Gel Against The Overview The Number of Fibroblasts in Healing Cuts Mice (Mus Musculus)
Abstract
Several sources cause skin tissue damage. Kefir probiotics, made from fermented cow's milk, are believed to remove scars during use. Kefir involves a series of immune processes, acute and chronic inflammatory processes, cell division cell migration, chemotaxis, and various differentiations. This research determines the histopathological fibroblast cells in the wound healings in mice (Mus musculus) given 80% kefir probiotic. The method of research was the posttest-only control group experimental design. Mus musculus aged eight weeks with a bodyweight of 18-40 grams were taken in two groups randomly with 32 Mice. The result is that fibroblasts in the negative control group had a smaller mean of 10,25 compared to the mean number of fibroblast cells in the 80% Kefir group, which was 25,18. Thus, it was proven that the effectiveness of Kefir probiotics of wound healing in mice (Mus musculus) was given 80% kefir probiotic (p=0,000 <0.05). There is an effect of kefir probiotic on the healing of cuts in mice based on the calculation of the number of fibroblast cells.
Keywords
References
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Baniasadi, M., Azizkhani, M., Saris, P. E. J., & Tooryan, F. (2021). Comparative antioxidant potential of kefir and yogurt of bovine and non-bovine origins. Journal of Food Science and Technology 2021, 1–10. https://doi.org/10.1007/S13197-021-05139-9
de Lima, M. dos S. F., da Silva, R. A., da Silva, M. F., da Silva, P. A. B., Costa, R. M. P. B., Teixeira, J. A. C., Porto, A. L. F., & Cavalcanti, M. T. H. (2018). Brazilian Kefir-Fermented Sheep’s Milk, a Source of Antimicrobial and Antioxidant Peptides. Probiotics and Antimicrobial Proteins, 10(3), 446–455. https://doi.org/10.1007/S12602-017-9365-8
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Prado, M. R. M., Boller, C., Zibetti, R. G. M., de Souza, D., Pedroso, L. L., & Soccol, C. R. (2016). Anti-inflammatory and angiogenic activity of polysaccharide extract obtained from Tibetan kefir. Microvascular Research, 108, 29–33. https://doi.org/10.1016/J.MVR.2016.07.004
Safitri, E. (2016). Pengaruh Pemberian Salep Kefir Terhadap Jumlah Sel Polimorfonuklear (Pmn) Dan Fibroblas Pada Kesembuhan Luka Sayatan Laparatomi Tikus (Rattus Norvegicus).
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Sulmiyati, Said, N. S., Fahrodi, D. U., Malaka, R., & Maruddin, F. (2019). The Physicochemical, Microbiology, and Sensory Characteristics of Kefir Goat Milk with Different Levels of Kefir Grain. Tropical Animal Science Journal, 42(2), 152–158. https://doi.org/10.5398/TASJ.2019.42.2.152
Wallace, H. A., Basehore, B. M., & Zito, P. M. (2021). Wound Healing Phases. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK470443/
Wilkinson, H. N., & Hardman, M. J. (2020). Wound healing: cellular mechanisms and pathological outcomes. Open Biology, 10(9). https://doi.org/10.1098/RSOB.200223
Yildiz, S. Addis, R., Cruciani, S., Santaniello, S., Bellu, E., Sarais, G., Ventura, C., Maioli, M., & Pintore, G. (2020). Fibroblast Proliferation and Migration in Wound Healing by Phytochemicals: Evidence for a Novel Synergic Outcome. International Journal of Medical Sciences, 17(8), 1030. https://doi.org/10.7150/IJMS.43986
Amita, K., & Balqis, U. (2017). GAMBARAN HISTOPATOLOGI PENYEMBUHAN LUKA SAYAT PADA MENCIT (Mus musculus) MENGGUNAKAN EKSTRAK DAUN BINAHONG (Anredera cordifolia (Tenore) Steenis) (Histophatological Finding of The Vulnus Incisivum Healing in Mice (Mus musculus) using Anredera cordifolia Leaf Extract). JURNAL ILMIAH MAHASISWA VETERINER, 1(3), 584–591. http://jim.unsyiah.ac.id/FKH/article/view/4143
Baniasadi, M., Azizkhani, M., Saris, P. E. J., & Tooryan, F. (2021). Comparative antioxidant potential of kefir and yogurt of bovine and non-bovine origins. Journal of Food Science and Technology 2021, 1–10. https://doi.org/10.1007/S13197-021-05139-9
de Lima, M. dos S. F., da Silva, R. A., da Silva, M. F., da Silva, P. A. B., Costa, R. M. P. B., Teixeira, J. A. C., Porto, A. L. F., & Cavalcanti, M. T. H. (2018). Brazilian Kefir-Fermented Sheep’s Milk, a Source of Antimicrobial and Antioxidant Peptides. Probiotics and Antimicrobial Proteins, 10(3), 446–455. https://doi.org/10.1007/S12602-017-9365-8
Dewi, M. L., Rusdiana, T., Muchtaridi, M., & Putriana, N. A. (2018). ARTIKEL TINJAUAN: MANFAAT KEFIR UNTUK KESEHATAN KULIT. Farmaka, 16(2), 80–86. https://doi.org/10.24198/JF.V16I2.18052
Dimidi, E., Cox, S. R., Rossi, M., & Whelan, K. (2019). Fermented Foods: Definitions and Characteristics, Impact on the Gut Microbiota and Effects on Gastrointestinal Health and Disease. Nutrients, 11(8). https://doi.org/10.3390/NU11081806
Farag, M. A., Jomaa, S. A., El-wahed, A. A., & El-seedi, H. R. (2020). The Many Faces of Kefir Fermented Dairy Products: Quality Characteristics, Flavour Chemistry, Nutritional Value, Health Benefits, and Safety. Nutrients, 12(2). https://doi.org/10.3390/NU12020346
Huseini, H. F., Rahimzadeh, G., Fazeli, M. R., Mehrazma, M., & Salehi, M. (2012). Evaluation of wound healing activities of kefir products. Burns, 38(5), 719–723. https://doi.org/10.1016/J.BURNS.2011.12.005
Johnson, T. R., Gómez, B. I., McIntyre, M. K., Dubick, M. A., Christy, R. J., Nicholson, S. E., & Burmeister, D. M. (2018). The Cutaneous Microbiome and Wounds: New Molecular Targets to Promote Wound Healing. International Journal of Molecular Sciences, 19(9). https://doi.org/10.3390/IJMS19092699
Knackstedt, R., Knackstedt, T., & Gatherwright, J. (2020). The role of topical probiotics on wound healing: A review of animal and human studies. https://doi.org/10.1111/iwj.13451
Lolou, V., & Panayiotidis, M. I. (2019). Functional Role of Probiotics and Prebiotics on Skin Health and Disease. Fermentation 2019, Vol. 5, Page 41, 5(2), 41. https://doi.org/10.3390/FERMENTATION5020041
Meire Dos Santos Falcão, de L., Roberto Afonso, da S., Milena Fernandes, da S., da Silva, P. A. B., Romero Marcos Pedrosa Brandão, C., José António Couto, T., Ana Lúcia Figueiredo, P., & Maria Taciana Holanda, C. (2018). Brazilian Kefir-Fermented Sheep’s Milk, a Source of Antimicrobial and Antioxidant Peptides. Probiotics and Antimicrobial Proteins, 10(3), 446–455. https://doi.org/10.1007/S12602-017-9365-8
Nam, Y., Kim, J. H., Baek, J., & Kim, W. (2021). Improvement of cutaneous wound healing via topical application of heat-killed lactococcus chungangensis cau 1447 on diabetic mice. Nutrients, 13(8). https://doi.org/10.3390/nu13082666
Oryan, A., Alemzadeh, E., & Eskandari, M. H. (n.d.). Kefir Accelerates Burn Wound Healing Through Inducing Fibroblast Cell Migration In Vitro and Modulating the Expression of IL-1ß, TGF-ß1, and bFGF Genes In Vivo. https://doi.org/10.1007/s12602-018-9435-6
Prado, M. R., Blandón, L. M., Vandenberghe, L. P. S., Rodrigues, C., Castro, G. R., Thomaz-Soccol, V., & Soccol, C. R. (2015). Milk kefir: composition, microbial cultures, biological activities, and related products. Frontiers in Microbiology, 6(OCT). https://doi.org/10.3389/FMICB.2015.01177
Prado, M. R. M., Boller, C., Zibetti, R. G. M., de Souza, D., Pedroso, L. L., & Soccol, C. R. (2016). Anti-inflammatory and angiogenic activity of polysaccharide extract obtained from Tibetan kefir. Microvascular Research, 108, 29–33. https://doi.org/10.1016/J.MVR.2016.07.004
Safitri, E. (2016). Pengaruh Pemberian Salep Kefir Terhadap Jumlah Sel Polimorfonuklear (Pmn) Dan Fibroblas Pada Kesembuhan Luka Sayatan Laparatomi Tikus (Rattus Norvegicus).
Salimi, F., & Mohammadipanah, F. (2021). Nanomaterials Versus The Microbial Compounds With Wound Healing Property. Frontiers in Nanotechnology, 2(January), 1–17. https://doi.org/10.3389/fnano.2020.584489
Sulmiyati, Said, N. S., Fahrodi, D. U., Malaka, R., & Maruddin, F. (2019). The Physicochemical, Microbiology, and Sensory Characteristics of Kefir Goat Milk with Different Levels of Kefir Grain. Tropical Animal Science Journal, 42(2), 152–158. https://doi.org/10.5398/TASJ.2019.42.2.152
Wallace, H. A., Basehore, B. M., & Zito, P. M. (2021). Wound Healing Phases. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK470443/
Wilkinson, H. N., & Hardman, M. J. (2020). Wound healing: cellular mechanisms and pathological outcomes. Open Biology, 10(9). https://doi.org/10.1098/RSOB.200223
Yildiz, S. C., Demir, C., Cengiz, M., & Ayhanci, A. (2019). Protective properties of kefir on burn wounds of mice that were infected with S. aureus, P. auroginasa and E. coli. Cellular and Molecular Biology, 65(7), 60–65. https://doi.org/10.14715/CMB/2019.65.7.11
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C., Demir, C., Cengiz, M., & Ayhanci, A. (2019). Protective properties of kefir on burn wounds of mice that were infected with S. aureus, P. auroginasa and E. coli. Cellular and Molecular Biology, 65(7), 60–65. https://doi.org/10.14715/CMB/2019.65.7.11
Zaetun, S., Analis, J., Poltekkes, K., Mataram, K., Rangkasari, J. P., Cermen, D., & Mataram, S. (2018). DAYA HAMBAT GETAH TANAMAN JARAK TINTIR (Jatropha Multifida Linn) TERHADAP PROSES PENYEMBUHAN LUKA DI TINJAU DARI HASIL PEMERIKSAAN CLOTTING TIME. Jurnal Kesehatan Prima, 8(2), 1308–1315. https://doi.org/10.32807/JKP.V8I2.52
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